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Nussbaum, R.L. et al. "Thompson & Thompson Genetics in Medicine," 6th edition (Revised Reprint), Saunders, 2004. Chapters 1- 6; Clinical Cases: Familial Hypercholesterolemia, Xeroderma Pigmentosum, Hemophilia, Thalassemia, I-Cell Disease, p. 215; Telomerase pp. 318-320.

Baynes, J. and Dominiczak, M.H. "Medical Biochemistry," (2nd edition) Mosby, 2004. Chapters 29-33.

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Learning Objectives for Lecture 10:

Understand the purpose for cloning DNA
Understand the characteristics of restriction enzymes and how they are useful for DNA cloning
Understand what cloning vectors are and how they are used to carry and amplify DNA inserts
Understand the basic differences between genomic and cDNA libraries
Understand how genomic libraries are constructed
Understand the purpose for having overlapping DNA fragments in genomic libraries and how they are generated
Understand how cDNA libraries are constructed and the use of reverse transcriptase for their construction
Understand the rationale for library screening
Understand the method of plaque hybridization
Understand the four methods for library screening and when they are put into use

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Lecture 7
Lecture 8
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Spring Semester

Molecular and Human Genetics
     Learning Objectives
     Lectures - Fall 2005
     Lectures - Spring 2006
     Internet Research Assignment

Medical Biochemistry

Lecture 10: DNA Cloning and Isolating Genes

9/12/05